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. 2021 Jun 25;12:695223. doi: 10.3389/fpls.2021.695223

Figure 2.

Figure 2

Yeast one hybridization. (A,B) Yeast one-hybrid (Y1H) of 12 transcription factors (TFs) with 11 monolignol pathway genes in Populus trichocarpa. TF-prey plasmids were transformed into bait strains and grown on SD/-ura-leu. We did not obtain transformants for VAL2a transformed into CCR2 promoter strain, and for MYB85a, BLH6b, BLH2, and MYB85b transformed into 4CL5 promoter strain (B). The transformants were selected on SD/-ura-leu+AbA (A). The concentrations of Aureobasidin A (AbA) are shown on right. (C–E) Y1H of 12 TFs with seven fragments of CAld5H2 promoter in P. trichocarpa. (C) The 2 kb P. trichocarpa CAld5H2 promoter was divided into seven fragments. Each fragment is about 300 bp long and two adjacent fragments have a 20–46 bp overlap. (D) Y1H assays of 12 TFs with fragments (3, 5, 6, and 7) of CAld5H2 promoters. The self-activation of fragments 1, 2, and 4 are too higher (1,000 μg/ml) to be removed. The transformants were grown on SD/-ura-leu+AbA for selection. The AbA concentrations were shown on the right. (E) The transformants were grown on SD/-ura-leu.