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. Author manuscript; available in PMC: 2022 Jul 8.
Published in final edited form as: Reproduction. 2021 Jul 8;162(2):129–139. doi: 10.1530/REP-21-0042

Fig 4. CEP164 expression in the male reproductive tract.

Fig 4.

(A) IF staining of ED sections for CEP164 and the lectin PNA. The boxed areas are magnified on the right. Scale bars, 10 µm and 5 µm (magnified images). Note that many sperm are typically found in the EDs of FoxJ1-Cre;CEP164 mice. (B) IF staining of testicular sections for CEP164 and the basal body marker G-tub. The boxed areas are magnified on the right. Scale bars, 10 µm and 1 µm (magnified images). (C) Quantification of the percentage of spermatids with CEP164 at G-tub-positive basal bodies is shown on the right. A total of 400 basal bodies were counted in S12 to S13 spermatids for each genotype (N=3 seminiferous tubules). Error bars represent means ± SD. ns, not significant. Nuclei were detected with DAPI. (D) Western blot analysis for CEP164 expression in the testis.