. 2021 Jul 9;16(7):e0253794. doi: 10.1371/journal.pone.0253794

Table 1. Summary data and reference intervals for hematology parameters of juvenile (< 5 mo) and adult (≥ 5 mo) Hartley guinea pigs.

Analyte	Age	N	Mean	SD	Median	Min	Max	P-value	Distribution	Method	RI	90% CI of LRL	90% CI of URL	P-value (Juveniles vs. Adults)
Hgb (g/dL)	Juveniles	67	15.2	0.7	15.1	13.4	16.7	0.371	G	NP	13.8–16.6	13.4–14.2	16.3–16.7	0.2668
Hgb (g/dL)	Adults	144	15.3	0.9	15.4	11.9	17.3	0.001	NG	NP	13.4–16.7	11.9–13.6	16.5–17.3	0.2668
Hgb (cell) (g/dL)	Juveniles	67	15.1	0.8	15.2	13.5	16.9	0.496	G	NP	13.6–16.6	13.5–13.9	16.2–16.9	0.2878
Hgb (cell) (g/dL)	Adults	144	15.2	1.0	15.4	11.9	17.4	0.002	NG	NP	12.9–17.0	11.9–13.4	16.6–17.4	0.2878
Hct (%)	Juveniles	67	46	2	46	41	52	0.012	NG	NP	41–52	41–42	50–52	0.0127^a
Hct (%)	Adults	144	47	3	47	38	53	0.000	NG	NP	41–51	38–42	51–53	0.0127^a
RBC (× 10⁶/μL)	Juveniles	68	5.59	0.32	5.56	4.76	6.53	0.599	G	NP	4.93–6.30	4.76–5.12	6.12–6.53	0.2247
RBC (× 10⁶/μL)	Adults	144	5.62	0.37	5.66	4.16	6.42	0.028	NG	NP	4.79–6.20	4.16–4.98	6.15–6.42	0.2247
MCV (fl)	Juveniles	68	83	3	83	77	90	0.062	G	NP	78–89	77–78	87–90	0.2222
MCV (fl)	Adults	143	84	3	84	78	94	0.000	NG	NP	79–89	78–80	88–94	0.2222
RDW (%)	Juveniles	67	13.0	1.6	12.5	11.0	19.4	0.000	NG	NP	11.1–17.2	11.0–11.2	15.4–19.4	0.0482^a
RDW (%)	Adults	143	13.0	0.9	12.9	11.1	16.0	0.000	NG	NP	11.5–15.3	11.1–11.7	15.0–16.0	0.0482^a
MCHC (g/dL)	Juveniles	68	33	1	33	31	35	0.000	NG	NP	31–35	31–32	34–35	0.0008^c
MCHC (g/dL)	Adults	144	32	1	32	30	35	0.000	NG	NP	31–34	30–31	34–35	0.0008^c
CHCM (g/dL)	Juveniles	68	33	1	33	30	35	0.000	NG	NP	31–35	30–31	34–35	0.0030^b
CHCM (g/dL)	Adults	143	32	1	32	30	35	0.000	NG	NP	31–34	30–31	34–35	0.0030^b
Platelets (× 10³/μL)	Juveniles	68	539	113	519	297	856	0.055	G	NP	332–801	297–363	735–856	0.0039^b
Platelets (× 10³/μL)	Adults	143	489	124	487	112	943	0.002	NG	NP	259–800	112–298	703–943	0.0039^b
MPV (fl)	Juveniles	68	8.0	0.7	7.9	6.6	9.2	0.040	NG	NP	6.8–9.1	6.6–7.0	9.0–9.2	< 0.0001^d
MPV (fl)	Adults	144	8.4	0.5	8.6	7.0	9.3	0.000	NG	NP	7.3–9.2	7.0–7.5	9.1–9.3	< 0.0001^d
WBC (× 10³/μL)	Juveniles	68	5.1	1.5	4.9	2.2	9.6	0.000	NG	NP	2.6–9.4	2.2–3.2	8.7–9.6	0.0018^b
WBC (× 10³/μL)	Adults	144	5.7	1.8	5.7	1.9	11.7	0.008	NG	NP	2.7–10.3	1.9–3.1	8.9–11.7	0.0018^b
Heterophils (%)	Juveniles	68	41	10	40	18	64	0.343	G	NP	18–61	18–26	56–64	< 0.0001^d
Heterophils (%)	Adults	132	49	11	49	23	79	0.380	G	NP	29–72	23–33	66–79	< 0.0001^d
Heterophils (× 10³/μL)	Juveniles	68	2.134	1.002	1.888	0.396	5.248	0.000	NG	NP	0.738–5.219	0.396–1.012	4.349–5.248	< 0.0001^d
Heterophils (× 10³/μL)	Adults	130	2.750	1.078	2.620	0.437	6.804	0.001	NG	NP	1.054–5.403	0.437–1.333	4.902–6.804	< 0.0001^d
Lymphocytes (%)	Juveniles	68	52	9	51	31	71	0.761	G	NP	33–71	31–38	68–71	< 0.0001^d
Lymphocytes (%)	Adults	132	41	12	41	14	70	0.243	G	NP	18–63	14–26	59–70	< 0.0001^d
Lymphocytes (× 10³/μL)	Juveniles	68	2.553	0.702	2.339	1.414	4.644	0.000	NG	NP	1.521–4.387	1.414–1.702	3.866–4.644	0.0357^a
Lymphocytes (× 10³/μL)	Adults	132	2.308	0.827	2.236	0.476	4.816	0.087	G	NP	0.775–4.177	0.476–1.140	3.577–4.816	0.0357^a
Foa-Kurloff Cells (%)	Juveniles	67	3	3	2	0	9	0.000	NG	NP	0–9	0–0	8–9	0.1032
Foa-Kurloff Cells (%)	Adults	132	3	2	3	0	11	0.000	NG	NP	0–9	0–0	8–11	0.1032
Foa-Kurloff Cells (× 10³/μL)	Juveniles	68	0.150	0.151	0.126	0	0.576	0.000	NG	NP	0–0.512	0–0	0.415–0.576	0.0504
Foa-Kurloff Cells (× 10³/μL)	Adults	132	0.192	0.163	0.156	0	0.757	0.000	NG	NP	0–0.710	0–0	0.500–0.757	0.0504
Monocytes (%)	Juveniles	68	4	2	3	0	9	0.000	NG	NP	0–9	0–1	8–9	0.3164
Monocytes (%)	Adults	131	4	3	4	0	14	0.000	NG	NP	0–10	0–1	8–14	0.3164
Monocytes (× 10³/μL)	Juveniles	67	0.172	0.124	0.159	0	0.558	0.000	NG	NP	0–0.503	0–0.033	0.415–0.558	0.0227^a
Monocytes (× 10³/μL)	Adults	131	0.239	0.195	0.205	0	1.020	0.000	NG	NP	0–0.792	0–0.019	0.696–1.020	0.0227^a
Eosinophils (%)	Juveniles	67	2	1	1	0	5	0.000	NG	NP	0–5	0–0	4–5	0.0106^a
Eosinophils (%)	Adults	130	2	2	2	0	8	0.000	NG	NP	0–7	0–0	6–8	0.0106^a
Eosinophils (× 10³/μL)	Juveniles	67	0.074	0.067	0.060	0	0.265	0.000	NG	NP	0–0.253	0–0	0.210–0.265	0.0048^b
Eosinophils (× 10³/μL)	Adults	128	0.117	0.101	0.102	0	0.442	0.000	NG	NP	0–0.363	0–0	0.330–0.442	0.0048^b
Basophils (%)	Juveniles	68	0	1	0	0	2	0.000	NG	NP	0–2	0–0	1–2	0.3021
Basophils (%)	Adults	130	0	1	0	0	2	0.000	NG	NP	0–2	0–0	2–2	0.3021
Basophils (× 10³/μL)	Juveniles	68	0.012	0.027	0	0	0.112	0.000	NG	NP	0–0.102	0–0	0.069–0.112	0.3041
Basophils (× 10³/μL)	Adults	128	0.016	0.030	0	0	0.108	0.000	NG	NP	0–0.102	0–0	0.089–0.108	0.3041

^aP < 0.05

^bP < 0.01

^cP < 0.001

^dP < 0.0001 between values for juvenile and adult Hartley guinea pigs. Abbreviations: G, Gaussian; NG, non-Gaussian; NP, nonparametric; RI, reference interval.