. 2021 Jun 24;26(13):3861. doi: 10.3390/molecules26133861

Table 1.

Characteristics of Timosaponin BII metabolites in gut microbiota by LC/MS-Q-TOF.

Metabolites	Retention Time (min)	Reaction *	Predicted Molecular Weight	Molecular Formula	Fragment Characteristics
Metabolites	Retention Time (min)	Reaction *	Predicted Molecular Weight	Molecular Formula	MS1/[M + H]⁺	MS/MS
M1	9.9	−Glc **	740.93	C₃₉H₆₅O₁₂	741	705, 579, 561, 435, 273, 255
M2	10.1	−Glc	740.93	C₃₉H₆₅O₁₂	741	705, 579, 561, 435, 273, 255
M3	10.2	−Glc	740.93	C₃₉H₆₅O₁₂	741	705, 579, 561, 435, 273, 255
M4	10.3	−2Glc	578.38	C₃₃H₅₄O₈	579	533, 435, 273, 255
M5	10.6	−2Glc	578.38	C₃₃H₅₄O₈	579	533, 435, 273, 255
M6	10.9	−2Glc-Gal ***	416.65	C₂₇H₄₄O₃	417	273, 255
M7	11.3	−2Glc-Gal	416.65	C₂₇H₄₄O₃	417	273, 255

* Reaction refers to the process to produce metabolites; ** Glc refers to the glucose moiety; *** Gal refers to the galactose moiety.