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. 2021 Jun 28;17(6):e1009703. doi: 10.1371/journal.ppat.1009703

Fig 3. Percentage of PrP glycosylation isoforms among AF4 fractions for 263K (red), HY (green) and DY (blue).

Fig 3

A) Percentage of di-, mono-, and un-glycosylated PrP present in detergent-solubilized BHs as calculated from immunoblots of three BHs for each strain (representative immunoblot depicted in Fig 1C). B) Percentage of di- (circles), mono- (triangles), and un-glycosylated (rhombus) PrP isoforms present in each AF4 fraction as calculated from NBH (empty symbols), 263K (red), HY (green), and DY (blue) immunoblots of three BHs for each strain (representative immunoblot shown in Fig 2B). The Rh (nm) of particles contained in fractions 16, 26, 36/38, and 52/54 are indicated in brackets. C) Percentage (average ± SE) of di- (top) and mono-glycosylated (bottom) PrP isoforms in the range of fractions where the glycoform ratio remains constant. Three BHs were analyzed for each prion strain. p-value *** ≤ 0.001.