Fig 6. Conformational stability of 263K and DY PrP^Sc particles ranging from 25 to 65 nm R_h.

A) Representative immunoblots of 263K (top) and DY (bottom) fractions incubated with increasing concentrations of SDS and digested with PK. 3F4 (dilution 1:3,000) was used as primary antibody. B) Densitometric analysis of 263K (top) and DY (bottom) of remaining PrP^res after incubation of AF4 fractions with increasing concentrations of SDS. 263K data was fitted to a sigmoidal function whereas DY data was fitted to a single decay hyperbolic function. Samples from three BH for each strain were analyzed. C) SDS concentration at which PrP^res reaches half of the maximum intensity as a function of particle size. Data represents average of 3 replicates and error bars represent SE.