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. 2021 Jul 9;7(28):eabg4574. doi: 10.1126/sciadv.abg4574

Fig. 2. Loss of UCHL1 enhances mitophagy.

Fig. 2

(A) Left: Immunoblot analysis of mitochondrial outer membrane (OM; MFN1 and TOM20) and inner membrane (IM; TIM23 and COXIV) proteins upon 20 μM CCCP treatment. Right: Relative quantification of the immunoblot band intensity of indicated mitochondrial proteins. n = 3. (B) Left: Confocal fluorescence images of Mtphagy Dye in HEK293 UCHL1 WT and KO cells upon 20 μM CCCP treatment. Green indicates lysosome, and red indicates mitophagy. Right: Number of Mtphagy Dye red dots per cell in HEK293 UCHL1 WT and KO cells upon 20 μM CCCP treatment. UCHL1 WT (n = 17) and KO (n = 12) without CCCP treatment (−). UCHL1 WT (n = 13) and KO (n = 13) with 20 μM CCCP treatment. Scale bar, 20 μm. Two-way ANOVA with Sidak’s multiple comparison test was used (B). All data were presented as means + SD.