Figure 2. TLR2 induces encephalitogenicity in Th17 cells.

(A) Incidence of EAE in mice following transfer of IL-17⁺ cells expanded with MOG35–55 alone, MOG_35–55 + IL-23, or MOG_35–55 + PAM3CSK4. *p < 0.01 (Fisher’s exact test). n = 4–16 pooled biological replicates per group.

(B) Mean clinical scores of EAE mice were assessed until the experimental endpoint. *p < 0.01 (2-way ANOVA).

(C) Mean cell numbers of CD4⁺, CD11B⁺, CD4⁺IL-17⁺, CD4⁺IFN-γ⁺, and CD4⁺IL-17⁺IFN-γ⁺ isolated from CNS tissues of individual mice.

(D) Mean cell numbers of the same populations isolated from spleens of EAE mice. Data are representative of 3 independent experiments and are presented as means ± SDs.