(A) Representative flow cytometry plots (IL-17 and IFN-γ) following 4-day differentiation of Th17(β) and Th17(IL-1/IL-23) cells transduced with RV-Mock or ectopic vector containing Ipcef1 (RV-IPCEF).
(B) Pooled IL-17 expression data of 3 independent experiments.
(C and D) Proliferation analysis following 4-day differentiation and transduction of Th17(β) cells (C) and Th17(IL-1/IL-23) cells (D).
(E) Frequency of viable cells was measured by viability staining of the aforementioned groups. Data are presented as means ± SDs. All data are representative of 3 independent experiments.