Skip to main content
. Author manuscript; available in PMC: 2021 Jul 9.
Published in final edited form as: Cell Rep. 2021 Jun 29;35(13):109303. doi: 10.1016/j.celrep.2021.109303

Figure 6. IPCEF impairs migration of pathogenic Th17 cells.

Figure 6.

(A) Th17 cells were polarized as indicated, followed by transduction with RV-Mock or RV-IPCEF. RV+ cells were then sorted, reactivated, and plated on a Transwell for 48 h to determine the number of cells migrating toward a CCL20 gradient. n = 3 technical replicates per group.

(B) The frequency of CCR6 expression in Th17 cells, as indicated, was assessed by flow cytometry, and CCR6+ frequencies were pooled for analysis.

(C–E) Th17 or iTreg cells were transduced and plated on a Transwell, as described in (A). The number of cells migrating toward CCL17 (C), CCL1 (D), or CCL19 (E) was then assessed. **p < 0.01, ***p < 0.001, ****p < 0.0001 (2-way ANOVA). Data are representative of 3 independent experiments and are presented as means ± SDs.