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. 2021 Jun 25;11:694145. doi: 10.3389/fonc.2021.694145

Figure 2.

Figure 2

TGF-β1 attenuates the cytotoxicity of T cells for HCC cells. (A) Activated T cells stimulated or not stimulated with TGF-β1 were co-cultured with SMMC-7721 cells or Bel-7404 cells for 24 h. CCK-8 assay was used to determine the cytotoxicity of T cells for SMMC-7721 and Bel-7404 cells. (B) LDH cytotoxicity assay was used to determine the cytotoxicity of T cells for SMMC-7721 and Bel-7404 cells. (C) Colony formation assay was used to determine the cytotoxicity of T cells for SMMC-7721 and Bel-7404 cells. n = 3, *P < 0.05, **P < 0.01 vs. control group.