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. 2021 Jun 25;11:694145. doi: 10.3389/fonc.2021.694145

Figure 4.

Figure 4

TGF-β1 upregulates the expression of PD-1 and CTLA-4 on CD8+ T cells and promotes HCC growth in vivo. (A) Flow cytometry was used to isolate CD3+ CD8+ T cells from peripheral blood mononuclear cells and detect the fluorescence intensity of PE-conjugated PD-1 protein and FITC-conjugated CTLA-4 protein on the cell surface. (B) Flow cytometry was used to detect the expression of PD-1 on CD8+ T cells in the peripheral blood of mice with HCC. (C) Flow cytometry was used to detect the expression of CTLA-4 on CD8+ T cells in the peripheral blood of mice with HCC. (D) Subcutaneous tumors extracted from Kunming mice 14 days after implantation, and the weight of these tumors. n = 3, *P < 0.05, **P < 0.01, ***P < 0.001 vs. control group.