Skip to main content
. 2021 Jun 9;10:e67681. doi: 10.7554/eLife.67681

Figure 1. Toxoplasma-injected neurons (TINs) show a predilection for the cortex at 3 weeks post-infection.

Cre-reporter mice were infected with II-Cre or III-Cre Toxoplasma parasites as indicated. Brains were harvested, sectioned, labeled, and quantified as previously described (Mendez et al., 2018). (A, B) Graphs of the absolute numbers of TINs mapped to 12 regions of the brain. (C, D) Graphs of TINs/region normalized to the size of the region. The dashed line is 1, the value at which the distribution of TINs would be considered proportional to the region size. Bars, mean ± SEM. N = 16–19/sections/mouse. Individual colors denote animals from individual cohorts, N = 4–12 mice/cohort for II-Cre infected mice, 2–11 mice/cohort for III-Cre infected mice. (C, D) *p=0.0170, **p=0.0021, ****p≤0.0001, one-sample t-test. p-Values for all regions are in Supplementary file 2. Mice were excluded from analyses if GFP+ cells were not above background rate of GFP+ cells in saline-injected Cre reporter mice or if identified as an outlier by ROUT outlier test, which exclude both Cohort 1 (red) III-Cre infected mice. Figure 1—figure supplement 1 includes all mice and uses all Allen Institute sections for normalization/enrichment index.

Figure 1—source data 1. Raw data for TINs count for II-Cre and III-Cre cohort 1 (Red).
elife-67681-fig1-data1.xlsx (403.9KB, xlsx)
Figure 1—source data 2. Raw data for TINs count for II-Cre and III-Cre cohort 2 (Green).
elife-67681-fig1-data2.xlsx (385.3KB, xlsx)
Figure 1—source data 3. Raw data for TINs count for II-Cre and III-Cre cohort 3 (Blue).
elife-67681-fig1-data3.xlsx (397.5KB, xlsx)
Figure 1—source data 4. Raw data for 10 mice each for TINs count for II-Cre and III-Cre cohort 4 (Black).
elife-67681-fig1-data4.xlsx (414.1KB, xlsx)
Figure 1—source data 5. Raw data for remaining mice for TINs count for II-Cre and III-Cre cohort 4 (Black).
elife-67681-fig1-data5.xlsx (381.3KB, xlsx)

Figure 1.

Figure 1—figure supplement 1. Original data with all mice and using full Allen Atlas area.

Figure 1—figure supplement 1.

Cre-reporter mice were infected with II-Cre or III-Cre Toxoplasma parasites as indicated. Brains were harvested, sectioned, labeled, and analyzed as described in Figure 1. (A, B) Graphs of the absolute numbers of Toxoplasma-injected neurons (TINs) mapped to 12 regions of the brain. (C, D) Graphs of TIN numbers/region normalized to the size of the region. The dashed line indicates the value at which TINs distribution would be considered random and appropriate for the region size. Data presented is before the exclusion of any mice. Exclusion was determined by (i) the total number of TINs being less than or equal to the total GFP+ central nervous system (CNS) cells in saline-injected mice or (ii) identification as an outlier via the ROUT method. Bars, mean ± SEM.
Figure 1—figure supplement 2. The visual, somatosensory, and motor cortices are highly enriched cortical regions containing Toxoplasma-injected neurons (TINs).

Figure 1—figure supplement 2.

(A) Graph showing the normalized distribution of cortical TINs from II-Cre infected mice in motor (MO), somatosensory (SS), and visual (VIS) cortices. (B) As in (A) except for III-Cre infected mice. *p≤0.05, **p=0.01, ***p≤0.0005, by one-sample t-test. Individual colors denote mice from the same cohort. The dashed line indicates the value at which TINs distribution would be considered random and appropriate for the region size. Lines, mean ± SEM.