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. 2021 Jun 9;10:e67681. doi: 10.7554/eLife.67681

Figure 4. Neuron with electorphysiology of a medium spiny neuron (MSN) co-localizes with DARPP32 staining.

Figure 4.

(A) Sample tracing from the shown neuron. Note the hyperpolarized resting membrane potential of −80 mV and the delayed time to the first action potential. (B) Image of patched neuron filled with neurobiotin. Arrowhead points to filled neuron. Dashed white line denotes biotin-filled blood vessels. (C) Image of DARPP32 staining. After recording and filling, the section was fixed and counter-labeled with anti-DARPP32 antibodies. (D) Merge of images B and C. (B–D) Section imaged on a Zeiss 880 confocal microscope. The shown images are a maximum projection of 12, 1 µm step images, from a 100 µm z-stack. Scale bar = 50 µm.