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. 2021 Jul 1;2021:9965099. doi: 10.1155/2021/9965099

Figure 1.

Figure 1

KDM1A increases the expression of PD-L1 in HCC cells. (a–c) Analysis of the clinical data of HCC patients from TCGA database. (a) The expression profile of KDM1A. (b) Association with overall survival of KDM1A. (c) Coexpression relationship of KDM1A and PD-L1. (d, e) mRNA expression (d) and protein expression (e) of KDM1A and PD-L1 in different cell lines. (f, g) Scheme of the gene type after the KDM1A (f) and PD-L1 (g) knockout in SMMC7721 cells. (h, i) Protein expression (h) and mRNA expression (i) of KDM1A and PD-L1 in wild-type SMMC7721 cells, SMMC7721KDM1A-/- cells, SMMC7721KDM1A-/- cells with KDM1A overexpression, and SMMC7721PD-L1-/- cells, and SMMC7721PD-L1-/- cells with PD-L1 overexpression. (j) ChIP-qPCR analysis of the PD-L1 promoter binding with H3K4me1/2/3 and H3K9me1/2/3 in wild-type SMMC7721 cells treated with the si-KDM1A or KDM1A expression plasmid. The data are presented as the means ± SD. n = 3 experiments in (a–i). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.01.