Table 1.
Primers used in the study.
| Bacteria | Gene name | Sequence (5′-3′) | Amplicon size expected (bp) | |
|---|---|---|---|---|
| Escherichia coli O157:H7 | Shiga toxins (stx1) | Forward | TTTGTYACTGTSACAGCWGAAGCYTTACG | 132 |
| Reverse | CCCCAGTTCARWGTRAGRTCMACRTC | |||
| Probe-FAM | CTGGATGATCTCAGTGGGCGTTCTTATGTA A | |||
| Shiga toxins (stx2) | Forward | TTTGTYACTGTSACAGCWGAAGCYTTACG | 128 | |
| Reverse | CCCCAGTTCARWGTRAGRTCMACRTC | |||
| Probe-VIC | TCGTCAGGCACTGTCTGAAACTGCTCC | |||
|
| ||||
| Listeria monocytogenes | hlyA-177 | hlyA-177-F | TGCAAGTCCTAAGACGCCA | 120 |
| hlyA-177-R | CACTGCATCTCCGTGGTATACTAA | |||
| hlyA-146 | hlyA-146-F | AAATCTGTCTCAGGYGATGT | 113 | |
| hlyA-146-R | CGATGATTTGAACTTCATCTTTTGC | |||
|
| ||||
| Salmonella enteritidis | invA | invA-F | CACGCTCTTTCGTCTGGCA | 154 |
| invA-R | TACGGTTCCTTTGACGGTGCGA | |||
|
| ||||
| Staphylococcus aureus | FemB | FemB-F | AATTAACGAAATGGGCAGAAACA | 93 |
| FemB-R | TGCGCAACACCCTGAACTT | |||