Table 2.

Known core attP sites of SPβ-related phages

Phage strain	Host	attP sequence (5′– > 3′)	attB sequence (5′– > 3′)	Integration locus
SPβ	B. subtilis 168	ACAGATAA/AGCTGTAT	ACAGATAA/AGCTGTAT	spsM
H2	B. amyloliquefaciens H	CCCTATAAATAACTA	CCCTATAAATAACTA	Intergenic
H2	B. subtilis 168	CCCTATAAATAACTA*	CCCttTAaAAATAACTA*	Intergenic*
φ3T	B. subtilis 168	aaaatgacataCCTACtgtgttttta	gctatgcggttCCTACctttgtcgtt	kamA
Goe11	B. subtilis ∆6	aaaatgacataCCTACtgtgtttttt*	gctatgcggttCCTACctttgtcgtt*	kamA*
Goe12	B. subtilis ∆6	ACAGATAA/AGCTGTAT*	ACAGATAA/AGCTGTAT*	spsM*
Goe13	B. subtilis ∆6	ACAGATAA/AGCTGTAT*	ACAGATAA/AGCTGTAT*	spsM*

The SPβ att sites were oriented based on Abe et al., 2017 [52]. Capital letters represent the inverted repeat recognised by SprA, a forward slash indicates the cleavage site, and underlined italic letters represent the 3' overhangs. The att sites of φ3T are presented as described by Suzuki et al., 2020 [55]. Capital letters indicate the conserved core, and lowercase letters indicate the associated imperfect repeat sequences. The att site sequences and integration loci marked with a star (*) are based on personal bioinformatic investigations and have not been confirmed experimentally.