Fig. 7.

Stress granules. a HeLa cells showed cytoplasmic SMPX-V5 foci that were positive for TIAL1. Scale bar = 20 µm. For other SG components in non-treated and MG132-treated cells, see Supplementary Fig. 2 (online resource). b, c Quantification of TIAL1 SGs in cells positive for SMPX-V5 (gray bars) and V5-negative cells in the same wells (white bars) after MG132 (b) or arsenite (c) stress. NT, non-treated; MG, 20 µM MG132 2 h; ARS, 500 µM sodium arsenite 45 min; R1/R3, recovery of 1 h / 3 h after stress. The graphs show mean ± SD of group means from 4 experiments. Asterisks indicates a significant difference to WT according to Dunnett’s multiple comparisons test (p = 0.0038; mean diff. –0.1737; 95% CI –0.2424 to –0.1050). For details, see Supplementary Tables 2–3 (online resource). d Delayed recovery of SMPX-enriched SGs. SGs from the V5-positive cells in (b) were classified as V5-enriched (dark bars) or non-enriched (light bars) and expressed as SG number per cell. For details, see Supplementary Fig. 2 and Supplementary Table 4 (online resource)