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. 2021 Jul 9;7:91. doi: 10.1038/s41523-021-00300-1

Fig. 5. The presence of JAB1 is required for AP1-driven transcription in TNBC cells.

Fig. 5

a BT549 or MDA-MB-231 cells were treated with 30 nM JAB1 siRNAs (siJAB1-1, siJAB1-2) or scrambled siRNA control for 3 days followed by western blot analysis to detect JAB1, cyclin D1, uPA, uPAR, and GAPDH with the respective antibodies. Data are the representative of two independent experiments. All blots derived from the same experiment and were processed in parallel. b BT549 or MDA-MB-231 cells were treated with 30 nM JAB1 siRNAs or scrambled siRNA control for 3 days followed by qRT-PCR to measure the levels of Cyclin D1, uPA and uPAR mRNA. Level of βActin mRNA was used for standardization. Data are means ± SD from three experiments. *P < 0.01 vs Control; #P < 0.005 vs Control. c, d BT549 or MDA-MB-231 cells were treated with 30 nM JAB1 siRNAs or scrambled siRNA control for 3 days and then subjected to RP-II (c) or JUN ChIP (d) followed by qPCR to analyze RP-II or JUN occupancy in cyclin D1, uPA and uPAR promoters. Data are means ± SD from three experiments. * P < 0.01 vs Control.