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. 2021 Jul 9;7:91. doi: 10.1038/s41523-021-00300-1

Fig. 6. Ser177 phosphorylation of JAB1 impacts JUN binding to AP1 sites.

Fig. 6

a BT549 or MDA-MB-231 cells were treated with 5 µM SB203580 for 1 day or 30 nM MK2 siRNA (siMK2-1) for 3 days for left untreated (control) and then subjected to JUN ChIP followed by qPCR to analyze JUN occupancy in cyclin D1, uPA and uPAR promoters. Data are means ± SD from three experiments. *P < 0.01 vs Control. b BT549 or MDA-MB-231 cells were transduced with JAB1D, JAB1A, or empty vector (control) for 3 days and then subjected to JUN ChIP followed by qPCR to analyze JUN occupancy in cyclin D1, uPA and uPAR promoters. Data are means ± SD from three experiments. *P < 0.01 vs Control. c, d BT549 (c) or MDA-MB-231 cells (d) were transduced JAB1D or empty vector for 3 days and then treated with 5 µM SB203580 for 1 day or 30 nM MK2 siRNA (siMK2-1) for 3 days followed by JUN ChIP to detect JUN occupancy in cyclin D1, uPA and uPAR promoters. Data are means ± SD from three experiments. *P < 0.01 vs Control.