Fig. 8. The presence of JAB1 is critical for tumor development.

a Luciferase-expressing MDA-MB-231 cells were transduced with lentiviral vector encoding either scramble or JAB1 shRNA for 3 days and then subcutaneously injected into nude mice. Tumor outgrowth was monitored weekly using the Xenogen IVIS-200 In Vivo bioluminescence imaging system. Data are means ± SD. n = 5. Statistical analysis was performed using Student t test (two-tailed) for the end experiment point. *P < 0.001. b Images of the xenograft tumors at 5 week. The image data is displayed in radiance or photons/sec/cm²/steradian. c Luciferase-expressing MDA-MB-231 cells were injected into nude mice for 5 days. Mice were randomized and treated daily with either Vehicle control or 1 µmol/kg of SB203580 i.p. for 4 weeks. Tumor growth were monitored weekly though Xenogen IVIS-200 in vivo imaging system. Data are means ± SD. n = 5. Statistical analysis was performed using Student’s t test (two-tailed) for the end experiment point. #P < 0.05. d Images of the xenograft tumors before treatment and after 4 weeks of treatment.