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. 2021 Jul 9;11:14186. doi: 10.1038/s41598-021-93616-8

Figure 7.

Figure 7

BMP2 as a novel target gene of miR-378a in keratinocytes. (a) Hypothetical binding structure of miR- 378a at 3′UTR of BMP2 (left) and INHBA (right) with predictive binding energy and probability value for actual binding. (b) Luciferase activity of wild-type and mutant 3′UTR of BMP2 genes in HEK293T cells co-transfected with miR-378a mimic or inhibitor for 48 h. (c) Western blot images (top) and quantitative measurement (bottom) of BMP2 protein in NHEK cells with miR-378a overexpression or suppression for 72 h. A representative image of BMP2 protein from four independent experiments was shown (also see Supplementary Fig. S5). (d) The expression level of BMP2 in psoriatic lesions (n = 4) and healthy individuals (n = 4). *Indicates P < 0.05, **indicates P < 0.01, and ***indicates P < 0.001.