Skip to main content
. 2021 Jul 9;12:4212. doi: 10.1038/s41467-021-24526-6

Fig. 1. CSR-1 isoforms have distinct spatial and temporal expression patterns.

Fig. 1

a Live imaging of CSR-1A (top) and CSR-1A + B (bottom) in an L4 hermaphrodite germline. At least three individual germlines were imaged for each strain. Scale bars, 25 μM. b Western blot detecting CSR-1 expression, using α-CSR-1 antibodies, in wild-type animals at L4 stage. c Western blot detecting for CSR-1A at each larval stage and gravid adult in 2xHA::mCherry::CSR-1A strain using α-HA antibody. Actin is shown as a loading control. d Schematic representation (top) of putative csr-1a and csr-1b promoters driving mCherry and GFP. The csr-1a promoter comprises ~1.5 kb of sequence preceding the first csr-1a exon, and the csr-1b promoter is the entire 544-bp intron between the unique csr-1a exon and the start codon of csr-1b. Schematic of the promoter reporter constructs created using the MosSCI system for csr-1a (middle) and csr-1b (bottom) are shown above images of gonads from L4 and gravid adult stages expressing the reporter constructs. Asterisk indicates intestinal autofluorescence visible near the bend in adult csr-1a::mCherry gonad. At least three individual germlines were imaged for each strain and condition. Scale bars, 25 μM. All blots have been reproduced. Source data are provided as a Source data file.