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. 2021 Jul 9;12:4208. doi: 10.1038/s41467-021-24535-5

Fig. 1. Hedgehog activation modifies mESC responsiveness to TGFβ signaling and improves ScxGFP induction.

Fig. 1

a Schematic of tendon differentiation protocol. Top row indicates common media components. Bottom row indicates media component variables. b, c Flow cytometry plots for ScxGFP induction under tenogenic media conditions. All conditions include FGF2. d Representative images of ScxGFP expression for tenogenic media conditions. All conditions include FGF2. All experiments were repeated a minimum of three times. Scale bar: 100 µm. e Flow cytometry quantification of ScxGFP induction for tenogenic media conditions (n = 4 independent samples, one-way ANOVA with Tukey’s post-hoc tests). All experiments were repeated a minimum of three times. Data represented as mean ± SD. f Counts histogram showing ScxGFP induction with increasing concentrations of TGFβ1 compared to TGFβ1 + SAG condition. g Real-time qPCR quantification of tendon and cartilage genes in sorted ScxGFPhi and ScxGFPlo cells (n = 6 independent samples, unpaired two-sided Student’s t-test). All experiments were repeated a minimum of three times. Data represented as mean ± SD. Bars indicate p < 0.05 between groups. *p < 0.05. Source data are provided as a source data file.