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. 2021 Jul 9;12(7):687. doi: 10.1038/s41419-021-03951-x

Fig. 3. SPP1 is increased in GECs in FSGS and contributes to angiogenic response.

Fig. 3

A Representative immunofluorescence images of SPP1 (red) with endothelial marker isolectin GS-IB4 (IB4, green, top panels) or podocyte marker, podocin (green, bottom panels). DNA is counterstained in blue. Scale bar, 50 μm. B Average fluorescence intensity of SPP1 in glomeruli of control vs. diseased mice are shown in arbitrary units (n = 6 control, 7 diseased mice, 20–30 glomeruli quantified per mouse). C Western blot analysis of cultured GECs expressing short harpin RNA (shRNA) against scrambled control (shScr) or against four independent clones of shRNA against Spp1 (ShSpp1-1, -2, -3, and -4). The densitometric analysis of SPP1 normalized to GAPDH is shown for shScr vs. shSpp1-2 (n = 3 independent experiments). D In vitro angiogenesis assay of GECs cultured in normal glucose (NG, 5 mM) or high glucose (HG, 25 mM) and plated on Matrigel bed. Images were taken 6 h post plating. Scale bar, 250 μM. **P < 0.01 between two groups by unpaired two-tailed t-test.