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. 2021 Jul 9;12(7):687. doi: 10.1038/s41419-021-03951-x

Fig. 6. Podocyte loss results in net GEC loss.

Fig. 6

A–D Representative immunofluorescence images of control and diseased glomeruli as indicated. Glomeruli are outlined in dotted lines. Scale bars, 50 μm. DNA is counterstained in blue. A Ki-67 (red) and endothelial marker isolectin GS-IB4 (IB4, green). Arrowheads show examples of Ki-67+ and IB4+ cells. B Phospho-p38 MAPK (p-p38, red) and IB4 (green). C DNA damage marker 8-oxo-2′-deoxyguanosine (oxo-dG, red) and IB4 (green). D Cleaved Caspase 3 (cl-Caspase 3, red) and EYFP marking the GECs. Arrowheads show examples of EYFP nuclei that are cl-Caspase 3+. DNA counterstain is omitted for clarity of double-positive nuclei in yellow. E Average number of EYFP+ cells per glomerular cross-section (n = 61 glomeruli from control mice, 53 from diseased mice, quantified from three mice per experimental group). P < 0.01 between groups by unpaired two-tailed t-test with Welch’s correction. F Average number of IB4/Ki-67 double-positive cells (left) and EYFP/cl-Caspase 3 double-positive cells per glomerular cross-section (gcs) in mice (n = 4 mice per group, 20–30 glomeruli evaluated per mouse). P < 0.001 and P < 0.0001 by unpaired two-tailed t-test.