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. 2021 Jul 9;12(7):691. doi: 10.1038/s41419-021-03973-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Knockdown of WDR4 expression in Huh-7 and HCC-LM3 cells by siRNA. B, C CCK-8 and colony formation assays showing the proliferation ability of Huh-7 and HCC-LM3 cells. D, E Flow cytometry results showing the effect of WDR4 knockdown on apoptosis and cell cycle progression in Huh-7 and HCC-LM3 cells. F The expression of genes related to the cell cycle and apoptosis was detected by Western blotting. *p < 0.05; **p < 0.01; ***p < 0.001.