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. 2021 Jul 9;12(7):691. doi: 10.1038/s41419-021-03973-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A, B CCK-8 and colony formation assays showing the proliferation ability of Huh-7 and HCC-LM3 cells. C, D Flow cytometry results showing the effect of CCNB1 on apoptosis and cell cycle progression. E, F The expression of genes related to the cell cycle and apoptosis was detected by Western blotting. G Transwell Matrigel invasion assay of cell invasion ability. H Scratch wound-healing motility assay of cell migration. I. The expression of genes related to EMT was detected by Western blotting. J Assessment of the size and weight of the dissected tumours. K Curves of tumour growth in each group. L Tumour weights were measured after collection. *p < 0.05; **p < 0.01; ***p < 0.001.