Fig. 4. Analysis of differential NVC dynamics in awake vs. anesthetized mice using TFs.

a Overlaid dynamics of Neuronal Ca²⁺ (GCaMP6 signal), RBC velocity (≥4th order capillaries), and penetrating arteriole diameter from all anesthetized experiments (10 vascular networks, 6 mice). b Mean traces of normalized data shown in a. c Transfer functions (TFs) are convolved with the average neuronal Ca²⁺ signal and optimized to predict the vascular response (RBC velocity or arteriole diameter). d TFs optimized to predict arteriole dilation (yellow) or RBC velocity (orange) dynamics computed with the mean anesthetized data. e TF predictions for arteriole diameter (top) and RBC velocity (bottom) from the experimental data from 3 different anesthetized experiments (TF_AN), top and bottom are paired experiments. f TFs optimized to predict arteriole dilation (blue) or RBC velocity (green) dynamics computed with the mean awake data (TF_AW). g Comparison of (TF_AW) and (TF_AN) prediction for arteriole diameter (top) and RBC velocity (bottom) from the experimental data from 3 different awake experiments, top and bottom are paired experiments. h Correlation of (TF_AW) and (TF_AN) with awake experimental data for RBC velocity (left) and arteriole diameter (right). i Comparison of rising slope for TF predictions made with (TF_AW) and (TF_AN) on awake data for RBC velocity (left) and arteriole diameter (right). The slope is defined as the D parameter of the 4-parameter sigmoidal fit formula: A + B/(1 + exp(−(−x− C) * D)). Data here are also represented in Figs. 1–3.