Fig. 3.

Expression of metabolic-related genes in control and 5-Aza treated CDCs. The relative mRNA expression of genes involved in glucose (GLUT1, GLUT4, PDK1 and PDK4) and fatty acid (CD36, CPT1B, PPARα, MCAT and ACADM) metabolism was assessed using qPCR in control and differentiated P2-CDCs created through FN/PDL or ColIV/HD. The mRNA expression was normalized to the geometric mean of Actb and β2M as reference genes and ColIV control as calibrator. Data are presented as mean ± SEM (n = 3), assessed using an ANOVA with Tukey post hoc test. Multicolour stars indicate multiple comparisons between groups and FN and ColIV control, black stars indicate comparison between control and differentiated cells. *p < 0.05; **p < 0.01; ***p < 0.001; **** p < 0.0001.