REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti GFP (Clones 7.1 and 13.1)	Roche	Cat#11 814 460 001; RRID: AB_390913
Mouse monoclonal anti V5	Invitrogen	Cat#R960-CUS; RRID: AB_2556564
Chicken Polyclonal anti GFP	Aves	Cat#GFP-1010; RRID: AB_2307313
Goat anti GFRα1	Neuromics	Cat#GT15004; RRID: AB_2307379
Goat Polyclonal α-GFP	ThermoFisher	Cat#A-11122; RRID: AB_221569
Goat polyclonal anti C-kit	R&D Systems	Cat#AF1356-SP
Rabbit polyclonal anti PLZF	Santa Cruz Biotechnology	Cat#sc-22839; RRID: AB_2304760
Rabbit polyclonal anti GFP	ThermoFisher	Cat#A11122; RRID: AB_221569
Mouse monoclonal anti Dcp1a	Sigma-Aldrich	Cat#WH0055802M6; RRID: AB_1843673
Chemicals, peptides, and recombinant proteins
TAG-NANOS2 (N-terminal tagged NANOS2 protein)	This paper	N/A
Critical commercial assays
SLAMseq Explorer Kit – Cell viability Titration Module	Lexogen	Cat#SKU: 059.24
SLAMseq Kinetics Kit – Catabolic Kinetics Module	Lexogen	Cat#SKU: 062.24
QuantSeq 3′ mRNA-Seq Library Prep Kit FWD	Lexogen	Cat#SKU: 015.24
SENSE Total RNA-Seq Library Prep Kit	Lexogen	Cat#SKU: 042.24
Ni-NTA Superflow	QIAGEN	Cat#30410
RNace-It Ribonuclease cocktail	Agilent	Cat#400720
RNasin Ribonuclease Inhibitor	Promega	Cat#N2115
Pierce spin columns snap cap	Thermo Scientific	Cat#69725
MetaPhor agarose	Lonza	Cat#50180
LA Taq	Takara	Cat#RR002M
MinElute Gel Extraction kit	QIAGEN	Cat#28604
Deposited data
CRAC-seq	This paper	GEO accession: GSE149835
RNA-seq	This paper	GEO accession: GSE149835
SLAM-seq	This paper	GEO accession: GSE149835
NANOS2 protein interactome in SSCs (IP-Mass spectrometry)	This paper	Table S5
Experimental models: Cell lines
Mouse spermatogonial stem cells: Nanos2CTL	This paper	N/A
Mouse spermatogonial stem cells: Nanos2TAG/+	This paper	N/A
Mouse spermatogonial stem cells: Nanos2TAG/"tnTAG	This paper	N/A
Mouse embryonic fibroblasts (feeders for SSCs)	This paper	N/A
Experimental models: Organisms/strains
Mouse: Nanos2TAG DBA/2J x C57Bl/6 hybrid	This paper (DBA/2J strain from Charles River Laboratories)	DBA/2J strain code: 625
Mouse: Nanos2TAG C57Bl/6	This paper	N/A
Recombinant DNA
V5-c-Myc-PS-His6X-EGFP-FRT-neo-FRT (PS is PreScission)	This paper	N/A
Software and algorithms
FastQC software	http://www.bioinformatics.babraham.ac.uk/projects/fastqc/	N/A
FASTX-collapser v0.0.14)	http://hannonlab.cshl.edu/fastx_toolkit/	N/A
pyCRAC	(Webb et al., 2014)	https://bitbucket.org/sgrann/pycrac
flexbar v3.4.0	(Dodt et al., 2012)	N/A
STAR v. 2.5.3a aligner	(Dobin et al., 2013)	N/A
Salmon v. 0.13.1 quasi-quantification	(Patro et al., 2017)	N/A
HTSeq v.0.11.2	(Anders et al., 2015)	N/A
bamCoverage v3.1.3 (deepTools package)	(Ramírez et al., 2016)	N/A
SAMtools v1.9	(Li et al., 2009)	http://www.htslib.org/
Novoalign v2.07.00	Novocraft	http://www.novocraft.com
Integrative Genomics Viewer	Broad Institute	http://software.broadinstitute.org/software/igv/; RRID:SCR_011793
python 2.7 Jupiter notebooks, python libraries (pandas v0.19.2, NumPy v1.16.0, scipy v1.2.0)	(Turowski et al., 2016).	update of gwide toolkit v0.5.27 https://github.com/tturowski/gwide
Bioconductor Limma Package	(Ritchie et al., 2015)	https://bioconductor.org/packages/release/bioc/html/limma.html
Bioconductor topGO package	Bioconductor	https://bioconductor.org/packages/release/bioc/html/topGO.html
stats R package	R project	https://www.r-project.org/
MEME-ChIP	Machanick and Bailey, 2011	https://meme-suite.org/meme/tools/meme-chip
SlamDunk pipeline	(Neumann et al., 2019)	https://github.com/t-neumann/slamdunk
MaxQuant LFQ algorithm	(Cox et al., 2014)	N/A
Perseus version 1.6.0.2	(Tyanova et al., 2016)	N/A
ingenuity-pathway-analysis IPA (QIAGEN Inc.)	(Krämer et al., 2014)	https://www.qiagenbio-informatics.com/products/ingenuity-pathway-analysis
Affinity designer	https://affinity.serif.com/en-gb/	N/A
FlowJo software	https://www.flowjo.com/solutions/flowjo/downloads	N/A
Fiji ImageJ.	https://imagej.net/Downloads	N/A