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. 2021 Apr 28;100(8):101207. doi: 10.1016/j.psj.2021.101207

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Producing presumptive germline chimeras with PGC injection and donor-derived progeny with backcrossing. (A) Incubation of Black Transylvanian naked neck recipient eggs for injection of donor PGCs. (B) Injection of Partridge color Hungarian donor PGCs into 2.5-day-old recipient embryos. (C) The hatched Black Transylvanian naked neck presumptive germline chimera chickens. (D) Mature Black Transylvanian naked neck presumptive chimaera chickens. E: Partridge color Hungarian donor breed. They were kept in individual cages for the backcrossing experiment. (E/1) Partridge color Hungarian rooster. (E/2) Partridge color Hungarian hen. F: Hatched chicks from the mating experiments. All four possible varieties are shown. (G) One-week-old donor-derived Partridge color chickens. (Photos by B. Lázár, E. Patakiné Várkonyi, I. Lehoczky and http://www.geneconservation.hu).