Figure 3.

Spaceflight changes transcriptome in F1 offspring

(A) Volcano plot of RNA-seq results in offspring liver collected from pGC and pMG mice (n = 5–6).

(B) Heatmap for expression (transcripts per million) of DEGs in offspring liver. DEGs overlapping with ATF7-target genes in GC testis are indicated by cluster name.

(C) Pathway analysis of DEGs upregulated by pMG using Reactome datasets. The top five pathways ranked by p value (Bonferroni correction for multiple testing) are presented with the gene number involved in each pathway.

(D) GSEA plot of upregulated genes in dividing cells. GSEA was performed against whole expression profiles in pGC and pMG liver.

(E) Venn diagram between ATF7-target genes in GC testis and DEGs upregulated by pMG.

(F) Expected model of affecting the transcriptome in offspring by paternal stress involved in spaceflight.