Antibodies |
|
Anti-H3K4me3 |
MBL |
Cat#MABI-0304 (lot:14004); RRID: AB_11123891 |
Anti-H3K9me2 |
MBL |
Cat#MABI-0317 (lot:16001) |
Anti-H3K9me3 |
MBL |
Cat#MABI-0318 (lot:16001) |
Anti-H3K27me3 |
MBL |
Cat#MABI-0323 (lot:15007); RRID: AB_11123929 |
Anti-ATF7 |
B.C. supplied |
2F10 |
|
Chemicals, peptides, and recombinant proteins |
|
Trizol |
Thermo Fisher |
Cat#15596018 |
Collagenase |
Sigma |
Cat#C6138 |
Trypsin |
Wako |
Cat#208-17251 |
DNase I |
Takara |
Cat#2270A |
BSA |
Sigma |
Cat#A7906 |
Percoll |
GE Healthcare |
Cat#17-0891-02 |
protein A-sepharose beads |
GE Healthcare |
Cat#17-0780-01 |
anti-mouse IgG-conjugated magnetic beads |
Thermo Fisher |
Cat#11201D |
RNase A |
Thermo Fisher |
Cat#EN0531 |
proteinase K |
Millipore |
Cat#1.24568 |
phenol:chloroform:isoamyl alcohol |
Wako |
Cat#311-90151 |
AMPure XP beads |
Beckman Coulter |
Cat#A63881 |
|
Critical commercial assays |
|
QuantiFast SYBR Green PCR Kit |
Qiagen |
Cat# 204056 |
NEBNext Ultra II DNA Library Prep Kit |
NEB |
Cat#E7645 |
Illumina NextSeq500 with High Output Kit v2 |
Illumina |
Cat#FC-404-2005 |
NEBNext Small RNA Library Prep Kit |
NEB |
Cat#E7300 |
|
Deposited data |
|
ChIP-seq data |
This paper |
DDBJ: DRA010655 (https://ddbj.nig.ac.jp/DRASearch/submission?acc=DRA010655) |
Small RNA-seq data |
This paper |
DDBJ: DRA010655 (https://ddbj.nig.ac.jp/DRASearch/submission?acc=DRA010655) |
refGene |
UCSC Table Browser |
https://genome.ucsc.edu/cgi-bin/hgTables |
Mouse tRNA sequence |
GtRNAdb |
http://gtrnadb.ucsc.edu/genomes/eukaryota/Mmusc10/mm10-tRNAs.fa |
Mouse miRNA sequence |
miRBbase |
http://www.mirbase.org/ (Release 22) |
|
Experimental models: organisms/strains |
|
Mouse: C57BL6/wild-type |
Jackson Laboratory |
N/A |
|
Oligonucleotides |
|
Primers for qChIP assay |
This paper |
Table S6 |
|
Software and algorithms |
|
CLC Genomics Workbench |
Qiagen |
10.1.1 |
BED tool |
Quinlan and Hall (2010) |
2.27.0 |
MACS2 |
Feng et al. (2012) |
2.1.1 |
deepTools2 |
Ramírez et al. (2016) |
3.1.2.0.1 |
PANTHER classification system |
Mi et al. (2013) |
14.0 |
SPOTS1.0 |
Shi et al. (2018) |
1.0.5 |
GSEA |
Subramanian et al.(2005) |
4.0.3 |
mirPath v.3 |
Vlachos et al. (2015) |
http://snf-515788.vm.okeanos.grnet.gr/ |