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. 2021 Jun 25;24(7):102773. doi: 10.1016/j.isci.2021.102773
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Anti-H3K4me3 MBL Cat#MABI-0304 (lot:14004); RRID: AB_11123891
Anti-H3K9me2 MBL Cat#MABI-0317 (lot:16001)
Anti-H3K9me3 MBL Cat#MABI-0318 (lot:16001)
Anti-H3K27me3 MBL Cat#MABI-0323 (lot:15007); RRID: AB_11123929
Anti-ATF7 B.C. supplied 2F10

Chemicals, peptides, and recombinant proteins

Trizol Thermo Fisher Cat#15596018
Collagenase Sigma Cat#C6138
Trypsin Wako Cat#208-17251
DNase I Takara Cat#2270A
BSA Sigma Cat#A7906
Percoll GE Healthcare Cat#17-0891-02
protein A-sepharose beads GE Healthcare Cat#17-0780-01
anti-mouse IgG-conjugated magnetic beads Thermo Fisher Cat#11201D
RNase A Thermo Fisher Cat#EN0531
proteinase K Millipore Cat#1.24568
phenol:chloroform:isoamyl alcohol Wako Cat#311-90151
AMPure XP beads Beckman Coulter Cat#A63881

Critical commercial assays

QuantiFast SYBR Green PCR Kit Qiagen Cat# 204056
NEBNext Ultra II DNA Library Prep Kit NEB Cat#E7645
Illumina NextSeq500 with High Output Kit v2 Illumina Cat#FC-404-2005
NEBNext Small RNA Library Prep Kit NEB Cat#E7300

Deposited data

ChIP-seq data This paper DDBJ: DRA010655 (https://ddbj.nig.ac.jp/DRASearch/submission?acc=DRA010655)
Small RNA-seq data This paper DDBJ: DRA010655 (https://ddbj.nig.ac.jp/DRASearch/submission?acc=DRA010655)
refGene UCSC Table Browser https://genome.ucsc.edu/cgi-bin/hgTables
Mouse tRNA sequence GtRNAdb http://gtrnadb.ucsc.edu/genomes/eukaryota/Mmusc10/mm10-tRNAs.fa
Mouse miRNA sequence miRBbase http://www.mirbase.org/ (Release 22)

Experimental models: organisms/strains

Mouse: C57BL6/wild-type Jackson Laboratory N/A

Oligonucleotides

Primers for qChIP assay This paper Table S6

Software and algorithms

CLC Genomics Workbench Qiagen 10.1.1
BED tool Quinlan and Hall (2010) 2.27.0
MACS2 Feng et al. (2012) 2.1.1
deepTools2 Ramírez et al. (2016) 3.1.2.0.1
PANTHER classification system Mi et al. (2013) 14.0
SPOTS1.0 Shi et al. (2018) 1.0.5
GSEA Subramanian et al.(2005) 4.0.3
mirPath v.3 Vlachos et al. (2015) http://snf-515788.vm.okeanos.grnet.gr/