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. 2021 Jun 27;26(13):3927. doi: 10.3390/molecules26133927

Table 3.

Nucleotide sequence of primers used for detection of rol-genes in hairy root cultures.

Genes Nucleotide Sequence (nt) Tm (°C)
rolA 5′-ggaaatccgcaatcaac-3′ 62
5′-tttgcacgcctaacaag-3′
rolB 5′-GCTCTTGCAGTGCTAGATTT-3′ 60
5′-GAAGGTGCAAGCTACCTCTC-3′
rolC 5′-CTCCTGACATCAAACTCGTC-3′ 60
5′-TGCTTCGAGTTATGGGTACA-3′
rolD 5′-CATCTGCAACTGAGCGTGTG-3′ 62
5′-TGTCTGATAGGGAGGAACGA-3′