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. 2021 Jun 29;118(27):e2106148118. doi: 10.1073/pnas.2106148118

Fig. 1.

Fig. 1.

DOT1L promotes the chromatin association of Pol II in human cells. (A and B) Comparison of the occupancies of total Pol II (A) and Pol II (ser-2p) (B) on an average gene in DOT1L KD versus control HEL cells by ChIP-seq. (C) Normalized read distribution of total and ser-2 phosphorylated Pol II ChIP-seq experiments within the c-MYC locus in DOT1L KD versus control HEL cells. (D) Characterization of a DOT1L KO K562 cell line by Western blot. (E) Comparison of total Pol II occupancies on an average gene in control and DOT1L KO cells. (F) Normalized read distribution of total Pol II ChIP-seq within the c-MYC locus in DOT1L KO versus control K562 cells. (G) A bar graph comparing Pol II occupancy changes on direct target and nontarget genes of DOT1L. Genes were divided into four groups according to their DOT1L binding status and their expression changes upon DOT1 loss as follows: group I: DOT1L-bound genes with expression changes; group II: DOT1L-bound genes without expression changes; group III: DOT1L-nonbound genes with expression changes; group IV: DOT1L-nonbound genes without expression changes. Genes with Pol II occupancies below the cutoff were included in the figure but not analyzed for occupancy changes.