Table 3.
Composition of media.
| Compound | Medium | |||||
|---|---|---|---|---|---|---|
| I * | II * | III * | IV | V | VI | |
| Apple pomace (kg/L) | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 |
| Potato wastewater (L) | - | - | 1.00 | - | - | 1.00 |
| Yeast extract (g/L) | - | 10.00 | - | - | 20.00 | - |
| Peptone (g/L) | - | 5.00 | - | - | 10.00 | - |
| Potassium dihydrogen phosphate (g/L) | - | 2.50 | - | - | 2.50 | - |
| Dipotassium hydrogen phosphate (g/L) | - | 1.50 | - | - | 1.50 | - |
| L-cysteine hydrochloride (g/L) | 0.40 | 0.40 | 0.40 | 0.40 | 0.40 | 0.40 |
| Biotin (mg/L) | - | 0.20 | - | - | 0.20 | - |
| Distilled water (L) | 1.00 | 1.00 | - | 1.00 | 1.00 | - |
| C/N ** | 36.6:1 | 4.6:1 | 3.6:1 | 34.9:1 | 4.4:1 | 3.5:1 |
* Extracts were diluted 2x to obtain media with a lower concentration of carbon sources. ** The calculations took into account glucose, fructose (reducing sugars), sucrose, and nitrogen/the study did not discuss the consumption of sucrose by the tested strain, because P. freudenreichii T82 does not assimilate and does not ferment this disaccharide.