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Silencing HDAC1 or HDAC2 using specific siRNA. The (A) NCI-H23 and (B) A549 cells were incubated for 48 h after transfection with 150 nM siH1 (siRNA targeting HDAC1), siH2 (siRNA targeting HDAC2), and scrambled RNA (scRNA). The levels of HDAC1 and HDAC2 expression were analyzed by Western blotting using anti-HDAC1 and anti-HDAC2 monoclonal antibodies. β-actin was used as the loading control.
