Table 1.
Total phenolics content (TPC), antioxidant capacity determinations, and total monomeric anthocyanin content (TMAC) of blackberry samples extracted using two different solvent systems 1.
| Samples 2 | TPC (mg GAE/100 g f.w.) 3 | H-ORACFL (μmol Trolox eq./100 g f.w.) 4 | FRAP (μmol Fe2+ eq./100 g f.w.) 5 | TMAC (mg C3G eq./100 g f.w.) 6 |
|---|---|---|---|---|
| methanol:water:hydrochloric acid (70.0/29.0/1.0, v/v/v) extraction | ||||
| MCE | 371.1 ± 19.0 b | 4458 ± 508 b | 2538 ± 150 b | 145 ± 4.7 b |
| MLF | 239.9 ± 4.8 b | 3498 ± 415 a | 1875 ± 101 a | – |
| MHF | 60.9 ± 0.9 b | 477 ± 47 b | 562 ± 36 b | – |
| acetone:water:acetic acid (70.0/29.5/0.5, v/v/v) extraction | ||||
| ACE | 433.8 ± 15.5 a | 6529 ± 560 a | 3403 ± 372 a | 134 ± 3.1 a |
| ALF | 171.6 ± 4.5 a | 3645 ± 299 a | 1886 ± 17 a | – |
| AHF | 121.0 ± 1.5 a | 1450 ± 70 a | 1113 ± 110 a | – |
1 Values for the crude methanolic extract and its fractions in each column with the same letter to the corresponding acetonic extract are not significantly (p > 0.05) different, as determined by Tukey’s multiple range test. All data are reported as means ± standard deviations (n = 9). 2 Abbreviations are as follows: LMW, low-molecular-weight; HMW, high-molecular-weight; MCE, methanolic crude extract; MLF, methanolic LMW fraction; MHF, methanolic HMW fraction; ACE, acetonic crude extract; ALF, acetonic LMW fraction; and AHF, acetonic HMW fraction. 3 TPC, total phenolics content; GAE, gallic acid equivalents; and f.w., fresh weight blackberries. 4 H-ORACFL, hydrophilic-oxygen radical absorbance capacity; eq., equivalents; and f.w., fresh weight blackberries. 5 FRAP, ferric reducing antioxidant power; eq., equivalents; and f.w., fresh weight blackberries. 6 TMAC, total monomeric anthocyanins content; C3G eq., cyanidin-3-O-glucoside equivalents; and f.w., fresh weight blackberries.