Table 2.
Quantitative determinations of dominant phenolic classes (mg/100 g f.w.) found in Georgia-grown ‘Ouachita’ blackberries based on the extraction solvent system employed 1.
| Phenolic Compounds Identified by HPLC–ESI–MS 2 |
Extraction Solvent System | |
|---|---|---|
| Methanolic | Acetonic | |
| Phenolic acids in MLF/ALF | ||
| protocatechuic acid hexoside | 0.57 ± 0.06 | 0.55 ± 0.03 |
| p-coumaric acid derivative | 0.60 ± 0.06 | 0.55 ± 0.10 |
| hydroxybenzoic acid hexoside | 0.39 ± 0.02 | 0.35 ± 0.03 |
| ellagic acid derivative | 1.41 ± 0.40 | 1.25 ± 0.16 |
| Total | 2.97 ± 0.54 | 2.71 ± 0.32 |
| Flavan-3-ols in MLF/ALF | ||
| (epi)catechin-4,8′-(epi)catechin hexoside | 15.26 ± 0.88 | 19.64 ± 0.82 |
| propelargonidin B-type dimer | 27.11 ± 1.56 | 31.80 ± 2.33 |
| Total | 42.37 ± 2.44 | 51.44 ± 3.15 |
| Anthocyanins in MLF/ALF | ||
| cyanidin-3-O-glucoside | 122.1 ± 7.4 | 99.88 ± 2.24 |
| cyanidin-3-O-arabinoside | 7.31 ± 1.20 | 6.83 ± 0.41 |
| cyanidin derivative | 5.69 ± 0.70 | 4.43 ± 0.45 |
| cyanidin-3-O-(6″-dioxalylglucoside) | 3.57 ± 0.49 | 3.22 ± 0.31 |
| Total | 138.7 ± 9.8 | 114.4 ± 3.4 |
| Flavonols in MLF/ALF | ||
| isorhamnetin derivative | 5.58 ± 0.32 | 4.62 ± 0.37 |
| quercetin-3-O-rutinoside | 4.62 ± 0.65 | 4.39 ± 0.52 |
| quercetin-3-O-galactoside | 7.68 ± 0.73 | 7.06 ± 0.14 |
| quercetin-3-O-glucoside | 4.25 ± 0.20 | 4.02 ± 0.02 |
| quercetin derivative | 5.10 ± 0.48 | 4.98 ± 0.39 |
| quercetin derivative | 5.16 ± 0.35 | 2.39 ± 0.22 |
| quercetin-3-O-acetylglucoside | 3.44 ± 0.20 | 1.64 ± 0.17 |
| Total | 35.83 ± 2.93 | 29.10 ± 1.83 |
| Ellagitannins in MHF/AHF | ||
| castalagin | 0.60 ± 0.10 | 1.47 ± 0.09 |
| lambertianin C isomer | 2.90 ± 0.50 | 5.47 ± 0.41 |
| sanguiin H-6 | 1.65 ± 0.18 | 2.37 ± 0.13 |
| Total | 5.15 ± 0.78 | 9.31 ± 0.63 |
1 Quantitation was performed using a Kinetex® XB-C18 column (4.6 × 150 mm i.d., 2.6-μm particle size, 100 Å; Phenomenex), and the data are reported as mg equivalents (eq.) of the available standard/100 g f.w. Commercial standards included protocatechuic acid, vanillic acid, (+)-catechin hydrate, cyanidin-3-O-glucoside chloride, and quercetin-3-O-rutinoside hydrate. The data are reported as means ± standard deviations (n = 3). 2 HPLC–ESI–MS, high-performance liquid chromatography–electrospray ionization–mass spectrometry.