Table 3.

Genomic neutral sites used earlier for the integration of expression cassettes in Synechocystis

Integration site	Replicon	Expressed protein/source organism	Reference
slr1311* (psbA2)	Chrom	Ipi/S. cerevisiae; CrtR, CrtP, CrtB/Synechocystis Pdc, Adh/Z. mobilis IspS/P. montana IspS/P. montana HmgS, HmgR/E. faecalis; AtoB/E. coli YFP	[88] [89] [59] [90] [91] [36]
slr0168* (N.A.)	Chrom	GFP/A. victoria, LuxAB/V. harveyi Ldh/B. subtilis, Sth/P. aeruginosa GFPmut3B	[92] [60] [93]
slr1181 (psbA1)	Chrom	Kivd, AdhA/L. lactis	[94]
sll1476 (N.A.)	Chrom	GFP/A. victoria	[65]
slr0573 (N.A.)	Chrom	GFP/A. victoria	[65]
slr1396 (N.A.)	Chrom	GFP/A. victoria	[65]
slr0271 (N.A.)	Chrom	GFP/A. victoria	[65]
slr0397 (N.A.)	Chrom	GFP/A. victoria	[65]
I.R.*	pCA2.4	YFP	[36]

N.A.: Not assigned; gene encoding protein of unknown function or annotated as hypothetical; Chrom.: Chromosome; I.R.*: Intergenic region between slr9101 and pCA24_1 (position 227—465 bp) in pCA2.4.; Ipi: isopentenyl diphosphate isomerase; CrtR: β-carotene hydroxylase; CrtP: phytoene desaturase; CrtB: phytoene synthase; Pdc: pyruvate decarboxylase; Adh: alcohol dehydrogenase II; IspS: isoprene synthase; HmgS: Hmg-CoA synthase; HmgR: Hmg-CoA reductase; AtoB: acetyl-CoA acetyl transferase; GFP: green fluorescent protein; LuxAB: luciferase; Ldh: L-lactate dehydrogenase; Sth: soluble transhydrogenase; GFPmut3B: synthetic green fluorescent protein; Kivd: 2-ketoisovalerate decarboxylase; AdhA: aldehyde reductase; YFP: yellow fluorescent protein. The integration sites used as controls in this study have been indicated by *