Table 1. RT-qPCR detail of COL-3 gene.
Gene symbols | Primer Sequence (5′ to 3′) Upper strand: sense Lower strand: antisense |
Product size (bp) | Annealing (°C) |
Cycle | References | |
---|---|---|---|---|---|---|
COL-3 | 5′-CCAGGAGCTAACGGTCTCAG -3′ 5′-CACGGTTTCCATCTCTTCCA -3′ |
103 | 54 | 40 | Ye et al., 2012 | |
β-Actin | 5′-AGACCTGTACGCCAACACAG-3′ 5′-TTCTGCATCCTGTCGGCAAT-3′ |
24 | 60 | 40 | Palumbo et al., 2018 |