Table 2.
Comparison of homogeneity, staining intensity, specificity and differentiation of immature bone, reversal and resting lines and canaliculi in normal and pathological bone in Hematoxylin and Eosin and kumkum stained sections
| Immature bone | Type of specimen | Homogeneity | Staining intensity | Specificity | Differentiation | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Negative | Poor | Good | Excellent | Negative | Poor | Good | Excellent | Negative | Poor | Good | Excellent | Negative | Poor | Good | Excellent | ||
| H and E | Normal bone | 0 | 0 | 0 | 10 | 0 | 0 | 3 | 7 | 0 | 0 | 4 | 6 | 0 | 8 | 2 | 0 |
| Pathological bone | 0 | 0 | 7 | 3 | 0 | 0 | 8 | 2 | 0 | 0 | 10 | 0 | 0 | 0 | 2 | 8 | |
| Kumkum stain | Normal bone | 0 | 0 | 1 | 9 | 0 | 0 | 0 | 10 | 0 | 0 | 0 | 10 | 0 | 0 | 0 | 10 |
| Pathological bone | 0 | 0 | 0 | 10 | 0 | 0 | 3 | 7 | 0 | 0 | 3 | 7 | 0 | 0 | 3 | 7 | |
| Z scores | Normal bone | −1.000 | −2.919 | −2.000 | −2.972 | ||||||||||||
| Pathological bone | −2.646 | −2.236 | −2.646 | −2.879 | |||||||||||||
| P (two-tailed) | Normal bone | 0.317 | 0.004 | 0.046 | 0.003 | ||||||||||||
| Pathological bone | 0.008 | 0.025 | 0.008 | 0.004 | |||||||||||||
| Reversal and Resting line | Type of specimen | Homogeneity | Staining intensity | Specificity | Differentiation | ||||||||||||
| Negative | Poor | Good | Excellent | Negative | Poor | Good | Excellent | Negative | Poor | Good | Excellent | Negative | Poor | Good | Excellent | ||
| H and E | Normal bone | 0 | 0 | 4 | 6 | 0 | 0 | 8 | 2 | 0 | 0 | 1 | 9 | 7 | 3 | 0 | 0 |
| Pathological bone | 0 | 0 | 7 | 3 | 0 | 7 | 3 | 0 | 4 | 6 | 0 | 0 | 8 | 2 | 0 | 0 | |
| Kumkum stain | Normal bone | 0 | 0 | 0 | 10 | 0 | 0 | 0 | 10 | 0 | 0 | 2 | 8 | 0 | 0 | 0 | 10 |
| Pathological bone | 0 | 0 | 0 | 10 | 0 | 0 | 0 | 10 | 0 | 0 | 0 | 10 | 0 | 0 | 0 | 10 | |
| Z scores | Normal bone | −2.000 | −2.828 | −2.913 | −2.919 | ||||||||||||
| Pathological bone | −2.646 | −2.889 | −2.889 | −2.972 | |||||||||||||
| P (two-tailed) | Normal bone | 0.046 | 0.005 | 0.004 | 0.004 | ||||||||||||
| Pathological bone | 0.008 | 0.004 | 0.004 | 0.003 | |||||||||||||
| Canaliculi | Type of specimen | Homogeneity | Staining intensity | Specificity | Differentiation | ||||||||||||
| Negative | Poor | Good | Excellent | Negative | Poor | Good | Excellent | Negative | Poor | Good | Excellent | Negative | Poor | Good | Excellent | ||
| H and E | Normal tooth | 0 | 1 | 4 | 5 | 0 | 2 | 8 | 0 | 7 | 3 | 0 | 0 | 7 | 3 | 0 | 0 |
| Pathological tooth | 0 | 1 | 7 | 2 | 1 | 9 | 0 | 0 | 9 | 1 | 0 | 0 | 9 | 1 | 0 | 0 | |
| Kumkum stain | Normal tooth | 0 | 0 | 2 | 8 | 0 | 0 | 1 | 9 | 0 | 0 | 0 | 10 | 0 | 0 | 1 | 9 |
| Pathological tooth | 0 | 0 | 2 | 8 | 0 | 0 | 0 | 10 | 0 | 0 | 3 | 7 | 0 | 0 | 0 | 10 | |
| Z scores | Normal tooth | −1.414 | −2.887 | −2.919 | −2.889 | ||||||||||||
| Pathological tooth | −2.646 | −3.051 | −2.889 | −3.051 | |||||||||||||
| P (two-tailed) | Normal tooth | 0.157 | 0.004 | 0.004 | 0.004 | ||||||||||||
| Pathological tooth | 0.008 | 0.002 | 0.004 | 0.002 | |||||||||||||
| Various pathologies | Type of specimen | Homogeneity | Staining intensity | Specificity | Differentiation | ||||||||||||
| Negative | Poor | Good | Excellent | Negative | Poor | Good | Excellent | Negative | Poor | Good | Excellent | Negative | Poor | Good | Excellent | ||
| H and E | Pathological tooth | 0 | 0 | 3 | 7 | 0 | 0 | 6 | 4 | 0 | 0 | 3 | 7 | 0 | 0 | 6 | 4 |
| Kumkum stain | Pathological tooth | 0 | 0 | 0 | 10 | 0 | 0 | 0 | 10 | 0 | 0 | 0 | 10 | 0 | 0 | 0 | 10 |
| Z scores | Pathological tooth | −2.919 | −2.000 | −2.919 | −2.000 | ||||||||||||
| P (two-tailed) | Pathological tooth | 0.004 | 0.046 | 0.004 | 0.046 | ||||||||||||
H and E: Hematoxylin and Eosin