Autosampler	LEAP (LEAP Technologies, Chapel Hill, NC) or Shimadzu SIL-20AC HT Prominence (Shimadzu, Columbia, MD)
HPLC pumps / Controller	Shimadzu CBM-20A or SCL-10A VP controller with LC-10/20AD pump (Shimadzu, Columbia, MD) and Eppendorf CH-30 column heater.
Autosampler Temp Settings	5°C
Column Type	DNAPac-PA-200, 4x250 mm, Part#: 063000 Vendor: Dionex, Cedar Rapids, IA
Column Temp Settings	60°C
Guard Column	Frit, change as needed
Flow Rate	1 mL/min
Injection Volume	30 μL
Mobile phase	A= 50:50 (1 mM EDTA in 25 mM Tis-HCl, pH 8.0): MeCN B= 50:50 (1 mM EDTA and 1.6 M NaCLO4 in 25 mM Tris-HCl, pH 8.0): MeCN (v/v) C= N/A
Needle Wash	1 = 50:50 (0.2% TEA and 0.02% EDTA in water): DMPF (v/v) 2 = 0.1% FA in water
LC Program	Gradient
Typical Column Pressure	160 bar
Detector	RF-20A xs fluorescence detector (Shimadzu, Columbia, MD)
Detector Temperature	20 °C