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Validation Parameters of the Method: Accuracy (Precision), Recovery Rates, and ME for the Quantification of NEH
| Intra-day assay (%) | |||||||
|---|---|---|---|---|---|---|---|
| Concentration [ng/mL] | Day 1 | Day 2 | Day 3 | Inter-day assay [%] | Recovery [%] (mean ± SD) | ME (CV) [%] | |
| 2.5 | 98.3 (5.7) | 113.5 (1.6) | 108.0 (1.0) | 107.7 (6.3) | 93.2 ± 2.8 | 118.1 (5.4) | |
| 50 | 104.5 (8.3) | 104.4 (3.5) | 110.0 (2.9) | 106.5 (4.8) | 96.9 ± 2.5 | 141.1 (4.7) | |
| 200 | 110.9 (1.5) | 109.8 (5.8) | 109.1 (3.2) | 109.9 (3.5) | 90.1 ± 1.6 | 105.2 (4.1) | |
