Figure 7.

Effects of the adenosine A₃R agonists Cl-IB-MECA and MRS5950 on voltage-gated Ca²⁺ currents in isolated DRG neurons. (A) Time course (left panel) and representative Ca²⁺ current traces (right panel) measured in a representative DRG neuron isolated from a DNBS-treated rat (DNBS) before and after the application of the A₃R agonist Cl-IB-MECA (30 nM) or the Ca²⁺ channel blocker Cd²⁺ (100 μM). (B) Averaged time courses of peak Ca²⁺ currents during Cl-IB-MECA (30 nM) superfusion in vehicle-treated or DNBS-treated animals in the absence or presence of the A₃AR antagonist, MRS1523 (1523: 100 nM). Pooled data (mean ± SEM) of % Ca²⁺ current inhibition induced by Cl-IB-MECA (C) or MRS5980 (D) applied alone or in combination with MRS1523 or PD173232 (PD; 1 μM) in vehicle-treated (white columns) or DNBS-treated (red columns) animals. In all panels: *P <0.05 vs respective control, paired Student t test. (E) Averaged time course of peak Ca²⁺ currents before or after MRS5980 (30 nM) superfusion DNBS rats in the presence of the A₃AR antagonist MRS1523 or the Ca²⁺ channel blocker PD173232 (1 μM). DRG, dorsal root ganglia.