Table 5.

Sequencing analysis of discrepant results for RRDR region of rpoB gene

Discrepant cases	Codons	Mutation
A	GGCACCAGCCAGCGGAGCCAATTCATGGACCAGAACAACCCGCTGTCGGGGTTGACCACAAGC GCCGACTGTCGGCGCTG	Leu 511Arg
B	GGCACCAGCCAGCTGAGCCAATTCATGGACCAGAACAACCCGCTGTCGGGGTTGACCCACAAGC GCCGACTGTCGGCGCTG	No mutation
C	GGCACCAGCCAGCTGAGCCAATTCATGTACCAGAACAACCCGCTGTCGGGGTTGACCCACAAGC GCCGACTGTCGGCGCTG	D516Y
D	GGCACCAGCCAGCTGAGCCAATTCATGGACCAGAACAACCCGCTGTCGGGGTTGACCTACAAGC GCCGACTGTCGGCGCTG	H526Y
E	GGCACCAGCCAGCTGAGCCAATTCATGGACCAGAACAACCCGCTGTCGGGGTTGACCTACAAGC GCCGACTGTCGGCGCTG	H526Y
F	GGCACCAGCCAGCTGAGCCAATTCATGTACCAGAACAACCCGCTGTCGGGGTTGACCCACAAGC GCCGACTGTTGGCGCTG	S531L
G	GGCACCAGCCAGCTGAGCCAATTCATGTACCAGAACAACCCGCTGTCGGGGTTGACCCACAAGC GCCGACTGTTGGCGCTG	S531L
H	GGCACCAGCCAGCTGAGCCAATTCATGGACCAGAACAACCCGCTGTCGGGGTTGACCCACAAGC GCCGACTGTCGGCGCTG	No mutation
I	GGCACCAGCCAGCTGAGCCAATTCATGGACCAGAACAACCCGCTGTCGGGGTTGACCCACAAGC GCCGACTGTCGGCGCTG	No mutation
J	GGCACCAGCCAGCTGAGCCAATTCATGTACCAGAACAACCCGCTGTCGGGGTTGACCCACAAGC GCCGACTGTTGGCGCTG	S531L
K	GGCACCAGCCAGCTGAGCCAATTCATGGACCAGAACAACCCGCTGTCGGGGTTGACCCACAAGC GCCGACTGTCGGCGCTG	No mutation

The 81-bp rifampicin resistant determining region (RRDR) region includes 27 codons (507–533). Sequences were analyzed with H37Rv sequence. Mutated codons are underlined. Amino acid changes are shown in right side of each aligned sequences.