Fig. 2.

β-GP induces the downregulation of RTEF-1 expression in human VSMCs and RTEF-1 genes with different expressed backgrounds are constructed in human VSMCs. Human aortic VSMCs were treated with or without 10 mM β-GP for 12 days (a and b, n = 3). RTEF-1-overexpressed VSMCs, RTEF-1 siRNA VSMCs ,or control VSMCs were constructed (c and d, n = 3). a RTEF-1 mRNA expression was determined by qRT-PCR. b RTEF-1 protein expression was analyzed by Western blot and quantified by densitometry. c RTEF-1 mRNA expression was detected in control VSMCs, RTEF-1 overexpressed VSMCs, negative siRNA VSMCs, or RTEF-1 siRNA VSMCs by qRT-PCR. d RTEF-1 protein expression was determined in control VSMCs, RTEF-1 overexpressed VSMCs, negative siRNA VSMCs, or RTEF-1 siRNA VSMCs by Western blot, and quantified by densitometry. Statistical significance was assessed using t-test. All graphs show mean + SD. ***P < 0.001 vs. Control. ^###P < 0.001 vs. NC siRNA