Fig. 7 |. βII-spectrin deficiency causes developmental and behavioral deficits.

a, E19 male embryos. b, Head circumference (n = 5). c, Eye distance (βII-SpWT (n = 7), βII-SpHet (n = 5), βII-SpKO (n = 5) of E19 embryos. Box plots show data points from minimum to maximum. Boxes represent data from the lower (25^th-percentile) to the upper (75^th-percentile) quartiles. Center and horizontal line inside a box indicate the 50^th-percentile and the median, respectively. Whiskers extends from the largest dataset number smaller than 1.5 times the interquartile range (IQR) to the smallest dataset number larger than 1.5IQR. One-way ANOVA with Tukey’s post hoc test. b, *P = 0.029, **P = 0.003. d, PND25 male mice. e, Body length at PND25. Data represent mean ± SEM (n = 12 mice/genotype). One-way ANOVA with Tukey’s post hoc test, ****P < 0.0001. f, Growth curve. Data represent mean ± SEM (n = 12 mice/genotype). g,h, Locomotor activity (g) and rearing (h) during an open-field test. Data in g and h represent mean ± SEM (n = 15 βII-SpWT, n = 5 βII-SpKO PND30 male mice). Data for f, g, i, and j were analyzed by Fisher’s PLSD tests following repeated measures ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Statistical comparisons were not conducted for h due to zero scores in the βII-SpKO group. i, j, Locomotor activity (i) and rearing (j) during an open-field test. k, Social preference during a three-chamber choice task. Within-genotype repeated measures ANOVA, *P = 0.0452. l, Entries into a chamber with a stranger mouse. Fisher’s PLSD test following repeated measures ANOVA, *P = 0.0306. Data in i–l represent mean ± SEM (n = 12 mice/genotype). See statistics summary in Source Data Figure 7.