Skip to main content
. 2021 Jan 12;26(10):5912–5924. doi: 10.1038/s41380-020-00992-0

Fig. 4. Injection of recombinant IL33 induces expression of a high level of p-AQP4 in astrocytes in aged Il33−/− brains.

Fig. 4

a Immunofluorescence shows a significant increase in p-AQP4 (red) expression in the cortex of Il33−/− mice after receiving rIL33 injection (Il33−/−+rIL33) as compared with PBS controls (Il33−/−+PBS); two-color staining (lower panels) reveals high density of p-AQP4 (red) but nearly no granular n-APQ4 around neurons (green, tubulinβ3) in Il33−/−+rIL33 mice, in contrast to many (arrows) in WT mice. Also note much less tubulinβ3 in both Il33−/−+rIL33 and Il33−/−+PBS mice than WT mice. b Immunofluorescence shows an increase in p-AQP4 expression in hippocampus (upper panels) of Il33−/−+rIL33 mice; note that GFAP+ astrocyte (green) population is similar among three groups (lower panel). c Western blot on cortical proteins shows quantity of AQP4 in Il33−/−+rIL33 mice is comparable to WT mice, and much higher than Il33−/−+PBS controls. d, e Statistics on total AQP4 expression (% of area) (d), and numbers of AQP4+ regions (e) in the cortex demonstrates that rIL33 injection enhances total AQP4 expression level but not numbers of AQP4+ regions.